Biomaterial Database

Purification of retinol-binding protein from serum and urine by affinity chromatography. 1978

G Fex, and B Hansson

A new method for the purification of retinol-binding protein from urine and serum is described. The method is based on the reversible binding of retinol-binding protein to retinoic acid linked to Sepharose and prealbumin linked to Sepharose. The yield is comparable to conventional methods using ion exchange and gel filtration and the product is an apo retinol-binding protein of high purity. The product has the same electrophoretic mobility and molecular weight and the same ability to interact with retinoic acid and prealbumin as retinol-binding protein prepared by conventional methods.

UI	MeSH Term	Description	Entries
D002846	Chromatography, Affinity	A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012177	Retinol-Binding Proteins	Proteins which bind with RETINOL. The retinol-binding protein found in plasma has an alpha-1 mobility on electrophoresis and a molecular weight of about 21 kDa. The retinol-protein complex (MW	Retinoid Binding Protein,Retinol Binding Protein,Retinoid Binding Protein, F-Type,Retinoid Binding Proteins,Retinol Binding Proteins,Binding Protein, Retinoid,Binding Protein, Retinol,Binding Proteins, Retinoid,Binding Proteins, Retinol,Protein, Retinoid Binding,Protein, Retinol Binding,Retinoid Binding Protein, F Type