-transparent.png){kind=logo}
Hamster embryos were treated with 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in vivo (in the mother) by transplacental application. The fetuses were isolated 24 h after administration of the AF-2 and cultured. Within the first 24h of primary culture, some parts of the cells were treated with colcemide for 3 h so that mitotic cells could be observed in the first cell cycle in vitro. Cultured embryonic fibroblasts in metaphase plates showed a marked dose-dependence in chromosomal aberrations. Transplacental application of AF-2 also caused slightly dose-dependent morphological transformation. When some transformed colonies were cloned and transferred to the hamster cheek pouch, these cells produced tumors in the host animals. This new in vivo--in vitro combination assay system is considered to be useful for detection of environmental potential carcinogens.
N Inui, and
Y Nishi, and
M Taketomi
November 1978,
Cancer letters,
N Inui, and
Y Nishi, and
M Taketomi
July 1983,
IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans,
N Inui, and
Y Nishi, and
M Taketomi
January 1982,
Eisei Shikenjo hokoku. Bulletin of National Institute of Hygienic Sciences,
N Inui, and
Y Nishi, and
M Taketomi
March 1977,
Biochemical and biophysical research communications,
N Inui, and
Y Nishi, and
M Taketomi
August 1977,
Gan,
N Inui, and
Y Nishi, and
M Taketomi
January 1994,
Mutation research,
N Inui, and
Y Nishi, and
M Taketomi
August 1975,
Mutation research,
N Inui, and
Y Nishi, and
M Taketomi
December 1977,
Gan,
N Inui, and
Y Nishi, and
M Taketomi
September 1993,
Biochemical pharmacology,
N Inui, and
Y Nishi, and
M Taketomi
February 1975,
The Tohoku journal of experimental medicine,
