Biomaterial Database

Relationship between bioassay and radioimmunoassay estimates of prolactin in rat serum. 1978

F C Leung, and S M Russell, and C S Nicoll

RIA and bioassay (BA) estimates of the concentration of PRL ([PRL]) in rat serum were compared using homologous assay systems. A rat mammary gland organ culture BA was developed which allowed objective and quantitative measurement of serum [PRL]. the serum samples were tested at two doses against three doses of the RP-1 rat PRL standard and the responses were quantified by a histometric procedure. The index of precision of the BA ranged from 0.1--0.4 and the secretory response was not affected by physiological concentrations of rat GH, T3, or by gonadal steroids. Serum samples taken from female rats during the estrous cycle, from suckled lactators, from rats bearing a PRL-secreting pituitary tumor, and from females that were treated with perphenazine were assayed. A highly significant correlation (r = 0.99) was obtained between the two assay methods with 21 samples. However, the regression coefficient was 4.20 +/- 0.55.Thus, although the two assays are highly correlated for measuring serum [PRL], the RIA measures only about 25% of the hormone that is detected by the mammary gland organ culture BA.

UI	MeSH Term	Description	Entries
D007774	Lactation	The processes of milk secretion by the maternal MAMMARY GLANDS after PARTURITION. The proliferation of the mammary glandular tissue, milk synthesis, and milk expulsion or let down are regulated by the interactions of several hormones including ESTRADIOL; PROGESTERONE; PROLACTIN; and OXYTOCIN.	Lactation, Prolonged,Milk Secretion,Lactations, Prolonged,Milk Secretions,Prolonged Lactation,Prolonged Lactations
D008321	Mammary Glands, Animal	MAMMARY GLANDS in the non-human MAMMALS.	Mammae,Udder,Animal Mammary Glands,Animal Mammary Gland,Mammary Gland, Animal,Udders
D009924	Organ Culture Techniques	A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)	Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D010546	Perphenazine	An antipsychotic phenothiazine derivative with actions and uses similar to those of CHLORPROMAZINE.	Chlorpiprazine,Perfenazine,Trilafon
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011388	Prolactin	A lactogenic hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). It is a polypeptide of approximately 23 kD. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation. Prolactin receptors are present in the mammary gland, hypothalamus, liver, ovary, testis, and prostate.	Lactogenic Hormone, Pituitary,Mammotropic Hormone, Pituitary,Mammotropin,PRL (Prolactin),Hormone, Pituitary Lactogenic,Hormone, Pituitary Mammotropic,Pituitary Lactogenic Hormone,Pituitary Mammotropic Hormone
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D012044	Regression Analysis	Procedures for finding the mathematical function which best describes the relationship between a dependent variable and one or more independent variables. In linear regression (see LINEAR MODELS) the relationship is constrained to be a straight line and LEAST-SQUARES ANALYSIS is used to determine the best fit. In logistic regression (see LOGISTIC MODELS) the dependent variable is qualitative rather than continuously variable and LIKELIHOOD FUNCTIONS are used to find the best relationship. In multiple regression, the dependent variable is considered to depend on more than a single independent variable.	Regression Diagnostics,Statistical Regression,Analysis, Regression,Analyses, Regression,Diagnostics, Regression,Regression Analyses,Regression, Statistical,Regressions, Statistical,Statistical Regressions
D004971	Estrus	The period in the ESTROUS CYCLE associated with maximum sexual receptivity and fertility in non-primate female mammals.
D005260	Female		Females