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Buck and Bodmer (1976) have developed a technique for identifying an antigen on the surface of human x mouse somatic cell hybrids, specified by a gene on a particular human chromosome. We have successfully adapted this technique to a study of marsupial cell surface antigens. Somatic cell hybrids between Macropus rufus (Marsupialia) lymphocytes and the mouse cell lines PG19 and 1R were injected intraperitoneally into mice of the same inbred strain from which the above cell lines were derived (C57B16J and C3H, respectively). The only identified M. rufus chromosome present in the hybrid cells was the X chromosome. The antisera, after adsorption with PG19 or 1R, were tested using indirect immunofluorescence, against the hybrid cells, and also against sub-clones (derived from hybrids) which had apparently lost the M. rufus X chromosome, or at least its long arm. The results of these tests showed that the absorbed antisera contained reactivity against an M. rufus cell surface antigen (or antigens). The reactions of one of the antisera were most simply interpreted by supposing that it was detecting an M. rufus X-lined antigen(s).
P J Sykes, and
R M Hope
January 1997,
Cytogenetics and cell genetics,
P J Sykes, and
R M Hope
January 1981,
Cytogenetics and cell genetics,
P J Sykes, and
R M Hope
December 1979,
Transplantation proceedings,
P J Sykes, and
R M Hope
April 1975,
Somatic cell genetics,
P J Sykes, and
R M Hope
July 1979,
Somatic cell genetics,
P J Sykes, and
R M Hope
November 1979,
Somatic cell genetics,
P J Sykes, and
R M Hope
January 1982,
Cytogenetics and cell genetics,
P J Sykes, and
R M Hope
March 1977,
Transplantation proceedings,
P J Sykes, and
R M Hope
November 1976,
Radiation research,
P J Sykes, and
R M Hope
May 1980,
Somatic cell genetics,
