Biomaterial Database

Uptake and esterification of palmitrate by rat diaphragm in vitro. 1968

G Schonfeld

The contribution of exogenously supplied palmitate to the intracellular palmitate pool and its role in esterification were studied for the intact rat diaphragm in vitro. Palmitate-1-(14)C attached to albumin in various molar ratios (nu) was taken up by the tissue in an initial rapid phase which led, after 4 min, to a steady-state level of tissue free fatty acid. The level was determined by nu but also by the albumin concentration below 2 g/100 ml. The exogenous palmitate taken up constitutes one-quarter to three-quarters of the intracellular palmitate pool as nu is increased from 0.5 to 5.5 (albumin concentration 2 g/100 ml). Turnover rates of intracellular palmitate at a given nu are stable over 35 min of incubation, as indicated by constant rates of esterification (largely to triglyceride, but also to phospholipids) and by the unchanging specific activity ratios between intracellular and medium palmitate-1-(14)C. These ratios also indicate that only a portion of the palmitate taken up is available for exchange with the albumin complex in the medium. Calculation of esterification rates at various nu values suggests that not all of the palmitate is in a pool that is available for esterification.

UI	MeSH Term	Description	Entries
D008041	Linoleic Acids	Eighteen-carbon essential fatty acids that contain two double bonds.	Acids, Linoleic
D008297	Male		Males
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009829	Oleic Acids	A group of fatty acids that contain 18 carbon atoms and a double bond at the omega 9 carbon.	Octadecenoic Acids,Acids, Octadecenoic,Acids, Oleic
D010169	Palmitic Acids	A group of 16-carbon fatty acids that contain no double bonds.	Acids, Palmitic
D002247	Carbon Isotopes	Stable carbon atoms that have the same atomic number as the element carbon but differ in atomic weight. C-13 is a stable carbon isotope.	Carbon Isotope,Isotope, Carbon,Isotopes, Carbon
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003964	Diaphragm	The musculofibrous partition that separates the THORACIC CAVITY from the ABDOMINAL CAVITY. Contraction of the diaphragm increases the volume of the thoracic cavity aiding INHALATION.	Respiratory Diaphragm,Diaphragm, Respiratory,Diaphragms,Diaphragms, Respiratory,Respiratory Diaphragms
D004952	Esters	Compounds derived from organic or inorganic acids in which at least one hydroxyl group is replaced by an –O-alkyl or another organic group. They can be represented by the structure formula RCOOR’ and are usually formed by the reaction between an acid and an alcohol with elimination of water.	Ester
D005230	Fatty Acids, Nonesterified	FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form.	Fatty Acids, Free,Free Fatty Acid,Free Fatty Acids,NEFA,Acid, Free Fatty,Acids, Free Fatty,Acids, Nonesterified Fatty,Fatty Acid, Free,Nonesterified Fatty Acids