Biomaterial Database

[Isolation and properties of lactate dehydrogenase isoenzymes from loach (Misgurnus fossilis) skeletal muscles and eggs]. 1979

R S Stoĭka, and S I Kusen'

The main lactate dehydrogenase (EC 1.1.1.27) isozyme from loach (Misgurnus fossilis) skeletal muscles was purified to homogeneity by polyacrylamide gel electrophoresis. The main group of lactate dehydrogenase isozymes which predominate in their activity in the unfertilized eggs of this teleost species and are stable to AgNO3 inhibition were partially purified. The effects of various concentrations of pyruvate, oxalate and urea on the activities of these purified enzyme preparations and their pH optima were studied. The antiserum for the purified lactate dehydrogenase isozyme from loach skeletal muscle was obtained. The decrease of the activity of this isozyme and that of the investigated group of isozymes from the eggs in the presence of increasing concentrations of antiserum was estimated.

UI	MeSH Term	Description	Entries
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D007770	L-Lactate Dehydrogenase	A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.	Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009928	Organ Specificity	Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.	Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D010063	Ovum	A mature haploid female germ cell extruded from the OVARY at OVULATION.	Egg,Egg, Unfertilized,Ova,Eggs, Unfertilized,Unfertilized Egg,Unfertilized Eggs
D005260	Female		Females
D005399	Fishes	A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia