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We have been studies time sequent stability each on standard human-C-peptide, human-C-peptide antiserum, 125I-tyrosyl human-C-peptide and the assay kit (all reagent) which is necessary in human proinsulin-C-peptide radioimmunoassay(RIA). Also we measured using this assay system, human proinsulin C-peptide in blood after oral administration of glucose to normal subject. Standard human-C-peptide and human-C-peptide antiserum were very stable on storage at 4 degrees C, 125I-tyrosyl human-C-peptide was unstable as compared with the former two, The stability of the assay kit was influenced by the stability of 125I-tyrosyl human-C-peptide, and was stable at 4 degrees C for ten weeks after preparation. Three lots of the assay kit prepared at different period showed almost same stability. We think this assay system using the assay kit is satisfactory in respect of stability. The measured values of human proinsulin-C-peptide in blood, using this assay system, showed insulin secretory reaction after oral administration of glucose.
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
April 1974,
Endocrinologia japonica,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
April 1973,
Endocrinologia japonica,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
November 1980,
Horumon to rinsho. Clinical endocrinology,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
January 1995,
Biochemistry and molecular biology international,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
November 1975,
Rinsho byori. The Japanese journal of clinical pathology,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
November 1989,
Radioisotopes,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
August 1974,
Kaku igaku. The Japanese journal of nuclear medicine,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
August 2005,
The FEBS journal,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
May 1986,
Clinical chemistry,
N Nakazawa, and
K Maki, and
H Ogawa, and
O Ikeda
September 1970,
Acta diabetologica latina,
