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For the purpose of estimating g-strophanthin radio-immunologically the following steps are described: the preparation of g-strophanthin antigen, the production of antibodies in the rabbit, the presentation of calibration curves, and the possibility of a quantitative determination of heterogenous glycosides. According to Malaprades reaction, g-strophanthin adheres to human albumin. With this antigen thus obtained antibodies are produced in rabbits. For the presentation of calibration curves, the addition of antiserum and g-strophanthin have to be matched in quantity; the calibration curves correspond to mathematical data. An increased sensitivity of the method is possible to attain by reducing the amount of labelled antigen and antiserum. The slight cross-reactivity of antibodies can be used to determine other glycosides when these are incubated labelled with tritium instead of 3H-g-strophanthin.
E Verspohl, and
H Strobach, and
K Greeff
February 1974,
Zeitschrift fur klinische Chemie und klinische Biochemie,
E Verspohl, and
H Strobach, and
K Greeff
January 1982,
Zentralblatt fur Gynakologie,
E Verspohl, and
H Strobach, and
K Greeff
July 1979,
Deutsche medizinische Wochenschrift (1946),
E Verspohl, and
H Strobach, and
K Greeff
May 1973,
Zeitschrift fur klinische Chemie und klinische Biochemie,
E Verspohl, and
H Strobach, and
K Greeff
January 1974,
Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. Albrecht von Graefe's archive for clinical and experimental ophthalmology,
E Verspohl, and
H Strobach, and
K Greeff
January 1976,
Arzneimittel-Forschung,
E Verspohl, and
H Strobach, and
K Greeff
April 1981,
Yao xue xue bao = Acta pharmaceutica Sinica,
E Verspohl, and
H Strobach, and
K Greeff
October 1975,
Clinica chimica acta; international journal of clinical chemistry,
E Verspohl, and
H Strobach, and
K Greeff
December 1973,
Zeitschrift fur klinische Chemie und klinische Biochemie,
E Verspohl, and
H Strobach, and
K Greeff
July 1978,
Der Urologe. Ausg. A,
