Biomaterial Database

Synaptosomal plasma membrane glycoproteins. II. Isolation of fucosyl-glycoproteins by affinity chromatography on the Ulex europeus lectin specific for L-fucose. 1977

J P Zanetta, and A Reeber, and G Vincendon, and G Gombos

After solubilization in sodium dodecyl sulphate, almost 90% of synaptosomal plasma membrane glycoproteins were separated from the bulk of synaptosomal plasma membrane proteins by sequential affinity chromatography on two immobilized lectins: concanavalin A and the Ulex europeus lectin specific for L-fucose. Four fractions were obtained and their sugar composition and electrophoretic patterns were determined. Fucosyl-glycoproteins contain more than 26% of protein and 85% of the protein-bound sugar of synaptosomal plasma membrane; hence they constitute a major class of glycoproteins in these membranes. The presence of some glucose in glycoproteins fractions obtained after affinity chromatography on the two lectins suggests that this sugar could be a structural component of some brain glycoproteins. Polyacrylamide gel electrophoresis revealed at least 28 major bands in fucosylglycoprotein fractions, and 11 in other fractions. Several of these major bands appear to contain more than one glycoprotein each. This heterogeneity appears to be mostly the result of the heterogeneity of the neuronal population in the central nervous system. Microheterogeneity of glycoprotein sugar chains and possible contamination of synaptosomal plasma membranes play, in our opinion, only a minor role.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002846	Chromatography, Affinity	A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D003208	Concanavalin A	A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
D005260	Female		Females
D005643	Fucose	A six-member ring deoxysugar with the chemical formula C6H12O5. It lacks a hydroxyl group on the carbon at position 6 of the molecule.	Deoxygalactose,alpha-Fucose,alpha Fucose
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006023	Glycoproteins	Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins.	C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013570	Synaptic Membranes	Cell membranes associated with synapses. Both presynaptic and postsynaptic membranes are included along with their integral or tightly associated specializations for the release or reception of transmitters.	Membrane, Synaptic,Membranes, Synaptic,Synaptic Membrane
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus