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A high pressure liquid chromatographic assay has been developed for the separation and quantitation of theophylline and its metabolites (1-methyl uric acid, 3-methyl xanthine, and 1,3-dimethyl uric acid), theobromine, and dyphylline in biological fluids, viz. human serum, urine, and saliva. The serum has been rendered protein-free by passage through a filter with a nominal molecular weight limit of 10,000. The filtrate is injected onto a reverse-phase column and the separation achieved by utilizing a polar mobile phase and dyphylline (dihydroxypropyl theophylline) as the internal standard. The results for two normal subjects are included to illustrate the applicability of the technique.
R K Desiraju, and
E T Sugita, and
R L Mayock
February 2000,
Journal of chromatography. A,
R K Desiraju, and
E T Sugita, and
R L Mayock
February 1996,
Journal of chromatography. B, Biomedical applications,
R K Desiraju, and
E T Sugita, and
R L Mayock
October 1974,
The Journal of laboratory and clinical medicine,
R K Desiraju, and
E T Sugita, and
R L Mayock
October 1994,
Analytical biochemistry,
R K Desiraju, and
E T Sugita, and
R L Mayock
November 1981,
Clinical chemistry,
R K Desiraju, and
E T Sugita, and
R L Mayock
August 1987,
Journal of chromatography,
R K Desiraju, and
E T Sugita, and
R L Mayock
December 1982,
Journal of chromatography,
R K Desiraju, and
E T Sugita, and
R L Mayock
January 1989,
Journal of pharmaceutical and biomedical analysis,
R K Desiraju, and
E T Sugita, and
R L Mayock
March 1988,
Journal of chromatography,
R K Desiraju, and
E T Sugita, and
R L Mayock
October 2000,
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan,
