BIOMAT Database Logo BIOMAT Database Logo

Chloroplast DNA from tobacco leaves. 1966

K K Tewari, and S G Wildman

DNA from tobacco leaf chloroplasts was isolated as a single component with a buoyant density in CsCl of 1.702 compared to 1.697 for nuclear DNA. 5-Methylcytosine is present in nuclear DNA but absent in chloroplast DNA. Chloroplast DNA, with a guanine-cytosine content of 43 percent, has a melting temperature of 86 degrees C and renatures completely on slow cooling, whereas nuclear DNA ( melting temperature, 84 degrees C; guanine-cytosine content, 40 percent) does not renature. About 9 percent of the total DNA in tobacco leaves is chloroplast DNA representing about 4.7 xX 10-(15) gram of DNA per chloroplast with a molecular weight of approximately 4 xX 10(7).

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D010947 Plants, Toxic Plants or plant parts which are harmful to man or other animals. Plants, Poisonous,Plant, Poisonous,Plant, Toxic,Poisonous Plant,Poisonous Plants,Toxic Plant,Toxic Plants
D002736 Chloroplasts Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA. Chloroplast,Etioplasts,Etioplast
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D014026 Nicotiana A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; the dried leaves of Nicotiana tabacum are used for SMOKING. Tobacco Plant,Nicotiana tabacum,Plant, Tobacco,Plants, Tobacco,Tobacco Plants

Related Publications

K K Tewari, and S G Wildman
Functional chloroplast polyribosomes from tobacco leaves.
March 1967, Science (New York, N.Y.),
K K Tewari, and S G Wildman
[Isolation of chloroplasts and chloroplast DNA from pea leaves].
January 1975, Biokhimiia (Moscow, Russia),
K K Tewari, and S G Wildman
Replication of chloroplast DNA of tobacco.
May 1966, Science (New York, N.Y.),
K K Tewari, and S G Wildman
A novel protein with DNA binding activity from tobacco chloroplast nucleoids.
September 1997, The Plant cell,
K K Tewari, and S G Wildman
Determination of the half-life of chloroplast transcripts in tobacco leaves.
January 2014, Methods in molecular biology (Clifton, N.J.),
K K Tewari, and S G Wildman
Buoyant density of tobacco and spinach chloroplast DNA.
April 1968, Biochimica et biophysica acta,
K K Tewari, and S G Wildman
Chloroplast DNA in mature and senescing leaves: a reappraisal.
March 2014, The Plant cell,
K K Tewari, and S G Wildman
PROTEIN SYNTHESIS BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. II. ASSOCIATION OF ACTIVITY WITH CHLOROPLAST RIBOSOMES.
May 1965, Biochemistry,
K K Tewari, and S G Wildman
Polysomes from expanded tobacco leaves.
October 1980, Planta,
K K Tewari, and S G Wildman
Ionones from cigar tobacco leaves.
June 2024, Journal of Asian natural products research,
Copied contents to your clipboard!