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A radioimmunoassay for plasma cortisol featuring the gamma-emitting radioligand 125I-iodohistamine, coupled to cortisol-3-(O-carboxymethyl)-oxime, is described. The new procedure retains much of the specificity associated with the use of anti-cortisol-3-BSA sera with tritium-labelled radioligands, and has the further advantages that running costs are lower and there is a greater potential for automation. Cortisol values obtained by this procedure agree well with those obtained by a published specific radioimmunoassay using the tritiated cortisol radioligand. Specificity of the procedure was checked by comparing values obtained with and without thin-layer chromatography purication: correlation was excellent (r = 0.96). Satisfactory levels of sensitivity, precision, and accuracy were obtained.
G F Read, and
D R Fahmy, and
R F Walker
November 1977,
Horumon to rinsho. Clinical endocrinology,
G F Read, and
D R Fahmy, and
R F Walker
August 1979,
Rinsho byori. The Japanese journal of clinical pathology,
G F Read, and
D R Fahmy, and
R F Walker
April 1980,
General and comparative endocrinology,
G F Read, and
D R Fahmy, and
R F Walker
April 1981,
Clinical chemistry,
G F Read, and
D R Fahmy, and
R F Walker
July 1975,
Horumon to rinsho. Clinical endocrinology,
G F Read, and
D R Fahmy, and
R F Walker
December 1995,
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies,
G F Read, and
D R Fahmy, and
R F Walker
November 1977,
Clinica chimica acta; international journal of clinical chemistry,
G F Read, and
D R Fahmy, and
R F Walker
September 1977,
Horumon to rinsho. Clinical endocrinology,
G F Read, and
D R Fahmy, and
R F Walker
May 1983,
Horumon to rinsho. Clinical endocrinology,
G F Read, and
D R Fahmy, and
R F Walker
October 1976,
Horumon to rinsho. Clinical endocrinology,
