Biomaterial Database

Tetrahymena: effect of freezing and subsequent thawing on breeding performance. 1967

E M Simon, and S W Whang

Tetrahymena pyriformis, syngen 1, frozen in 10 percent dimethyl sulfoxide, stored for 2 months, and then thawed could conjugate normally. More significantly, they were viable, in normal numbers, through two sex ual reorganizations. The strains ap parently did not sustain genetic dam age during the treatment. The tech niques offer considerable promise for the maintenance of breeding stocks in ciliated protozoans.

UI	MeSH Term	Description	Entries
D001947	Breeding	The production of offspring by selective mating or HYBRIDIZATION, GENETIC in animals or plants.	Breedings
D004121	Dimethyl Sulfoxide	A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.	DMSO,Dimethyl Sulphoxide,Dimethylsulfoxide,Dimethylsulphinyl,Dimethylsulphoxide,Dimexide,Rheumabene,Rimso,Rimso 100,Rimso-50,Sclerosol,Sulfinylbis(methane),Rimso 50,Rimso50,Sulfoxide, Dimethyl,Sulphoxide, Dimethyl
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D013768	Tetrahymena	A genus of ciliate protozoa commonly used in genetic, cytological, and other research.	Tetrahymenas