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Phytohemagglutinin (PHA)-induced stimulation of uridine incorporation into RNA was studied with cotton-wool isolated peripheral blood human lymphocytes. The amount of labeling in 24-hour cultures proved to be increased in men and in 45,XO patients, compared to women. With high doses of PHA applied, hyperreactivity of the 45, XO cells was revealed. PHA-activation in normal persons tends to decrease with advancing age. Complex genomic as well as sex hormone effects may be responsible for the differences observed.
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