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1. The inorganic-pyrophosphatase activity of alkaline phosphatases prepared from human liver and small intestine was investigated at different stages of purification. 2. Both liver and intestinal preparations possessed pyrophosphatase activity at all stages of purification, and the two types of activity were not separated by gel filtration or by anion-exchange or cation-exchange chromatography. 3. After starch-gel electrophoresis of the tissue extracts, the zones of pyrophosphatase activity coincided exactly with alkaline-phosphatase zones. 4. Hydrolysis of each type of substrate was inhibited by the presence of the other, and a constant ratio of alkaline-phosphatase activity to pyrophosphatase activity was maintained during inactivation of the enzymes by incubation at 55 degrees . 5. These results are consistent with the view that alkaline phosphatases are also inorganic pyrophosphatases.
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
January 1968,
Biochimica et biophysica acta,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
October 1967,
The Biochemical journal,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
October 1972,
Experimental cell research,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
July 1971,
Enzymologia,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
July 1973,
Biochimica et biophysica acta,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
October 1977,
Clinical orthopaedics and related research,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
January 1967,
Helvetica physiologica et pharmacologica acta,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
February 1986,
Journal of dental research,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
July 1994,
Nihon Seikeigeka Gakkai zasshi,
D W Moss, and
R H Eaton, and
J K Smith, and
L G Whitby
October 1971,
The Biochemical journal,
