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A controlled rate liquid nitrogen cooling device suitable for freeze preservation of living cells. 1967

P J Walker, and M J Wilén

UI MeSH Term Description Entries
D008297 Male Males
D009584 Nitrogen An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
D011309 Preservation, Biological The process of protecting various samples of biological material. Biological Preservation,Preservation, Biologic,Biologic Preservation
D005615 Freezing Liquids transforming into solids by the removal of heat. Melting
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012661 Semen The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma. Seminal Plasma,Plasma, Seminal
D014345 Trypanosoma A genus of flagellate protozoans found in the BLOOD and LYMPH of vertebrates and invertebrates, both hosts being required to complete the life cycle. Nannomonas,Trypanosomes,Nannomona,Trypanosome

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Statically controlled cooling rate device.
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Preservation of living cells by freeze-drying.
April 1960, Annals of the New York Academy of Sciences,
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[Deep-freeze preservation of fungi in liquid nitrogen as a basis for standardized inocula].
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[A digital controlled freeze drying device for electron microscopic preparation with extreme economic consumption of liquid nitrogen (author's transl)].
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Virulence of Trichomonas vaginalis after freeze-preservation for 2 years in liquid nitrogen vapor.
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