-transparent.png){kind=logo}
The development of a suitable bone marrow derived adherent cell population appears to be essential for the prolonged maintenance of haemopoietic stem cells in vitro. When established adherent layers are inoculated with freshly isolated bone marrow cells, proliferation of stem cells (CFU-S) regularly occurs both in the adherent layer and amongst the non-adherent cells. Furthermore, CFU-S present within the adherent layer are able to regenerate both themselves and the "non-adherent" CFU-S. One day after re-feeding the cultures (by removal of half the growth medium and addition of fresh medium) both the "non-adherent" and the "adherent" CFU-S are in a high cycling state (greater than 40% kill with 3HTdR). This proportion decreases with time of re-feeding and 5-7 days later the majority of "adherent" and "non-adherent" CFU-S are in a low cycling state ( less than 10% 3HTdR kill). Following a further re-feeding, CFU-S again enter a high cycling state.
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
January 1991,
Biotechnology (Reading, Mass.),
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
May 1987,
Cell and tissue kinetics,
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
November 1988,
Cell and tissue kinetics,
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
March 1985,
Biulleten' eksperimental'noi biologii i meditsiny,
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
February 1978,
Blood,
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
March 2006,
Blood,
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
January 1984,
Bibliotheca haematologica,
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
January 1978,
Scandinavian journal of haematology,
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
January 1999,
Stem cells (Dayton, Ohio),
T M Dexter, and
E G Wright, and
F Krizsa, and
L G Lajtha
July 1980,
Blood,
