-transparent.png){kind=logo}
A specific and sensitive radioimmunoassay for human pregnancy-associated plasma protein A (PAPP-A) is described. This assay utilized tracer repurified by affinity chromatography on heparin-Sepharose. The most sensitive assay developed had a detection limit of 5 microliters PAPP-A in serum, and this protein could not be detected in sera from non-pregnant subjects. During pregnancy, mean circulating levels rose from 0.6 mg/l at 6 weeks gestation to 110 mg/l at term, and PAPP-A was detected in 44 of 101 umbilical blood samples. The use of the anticoagulants EDTA and acid citrate dextrose resulted in significantly different results in the plasma compared to the corresponding serum sample. PAPP-A was also measured in amniotic fluid.
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
June 1981,
Biology of reproduction,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
July 1989,
British journal of obstetrics and gynaecology,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
November 2023,
Endocrine reviews,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
January 1982,
Clinical chemistry,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
January 2014,
Advances in clinical chemistry,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
January 1982,
Placenta. Supplement,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
July 1979,
Clinica chimica acta; international journal of clinical chemistry,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
October 2000,
Biochimica et biophysica acta,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
July 1989,
British journal of obstetrics and gynaecology,
L G Pinto Furtado, and
A E Bolton, and
J G Grudzinskas, and
M G Chapman, and
M J Sinosich, and
V Sharma
September 1982,
British journal of obstetrics and gynaecology,
