-transparent.png){kind=logo}
These assessment of the consequences of irradiation on chromatin is complicated by endogenous nucleases. Isolation and prolonged storage of rat liver nuclei in buffers containing divalent metal ions activates these enzymes and promotes the degradation of chromatin. Irradiation of rat liver nuclei to dose levels of 20,000 rad under conditions in which endogenous nucleases are inhibited and analysis of the irradiated chromatin by sucrose density gradient centrifugation gave no evidence for monosomes or oligosomes. When chromatin from irradiated nuclei was digested with micrococcal nuclease, the levels of monosomes and oligosomes were identical to those of micrococcal nuclease, the levels of monosomes and oligosomes are identical to suggest that irradiation results in neither a direct fragmentation of linkers nor the sensitization of linkers for subsequent cleavage by micrococcal nuclease. Histones isolated from monosomes of irradiated and unirradiated nuclei were intact, showing no fragmentation or loss of residues, as judged by sodium dodecyl sulfate-polyacrylamide electrophoresis.
D Gelderblom, and
B J Smit, and
L Böhm
January 1978,
Radiobiologiia,
D Gelderblom, and
B J Smit, and
L Böhm
August 1987,
Molecular and cellular biochemistry,
D Gelderblom, and
B J Smit, and
L Böhm
November 1986,
Biochemistry international,
D Gelderblom, and
B J Smit, and
L Böhm
June 1997,
Biochemistry. Biokhimiia,
D Gelderblom, and
B J Smit, and
L Böhm
January 1998,
Ukrainskii biokhimicheskii zhurnal (1978),
D Gelderblom, and
B J Smit, and
L Böhm
January 1981,
Radiobiologiia,
D Gelderblom, and
B J Smit, and
L Böhm
February 1976,
Biochimica et biophysica acta,
D Gelderblom, and
B J Smit, and
L Böhm
January 1983,
Radiobiologiia,
D Gelderblom, and
B J Smit, and
L Böhm
April 1986,
Biulleten' eksperimental'noi biologii i meditsiny,
D Gelderblom, and
B J Smit, and
L Böhm
December 1977,
The Indian journal of medical research,
