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Simultaneous isolation of two platelet membrane subfractions was achieved by centrifugation on 40% sucrose from a 100.000 g crude membrane fraction. Characterization of both types of membranes was carried out by different biochemical and immunological markers. Using a surface label, 3H Concanavalin A (3HCon A), a marker enzyme, phosphodiesterase, and lipid analysis, one of the fraction has been identified as external or plasma membranes, the other consists of intracellular membranes. Further two specific antibodies directed against external membrane antigens (LeKa and IgG L) react almost exclusively with the external membranes. Finally both kinds of membranes were able to uptake calcium but the affinity for this cation was higher for the internal than for the external membranes. This suggests that both membranes are implicated in the regulation of the cytoplasmic calcium concentration and that the internal membranes (dense tubular system) play the major part in this regulation.
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
January 1998,
Methods in molecular biology (Clifton, N.J.),
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
January 1981,
Thrombosis research,
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
March 1988,
Plant physiology,
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
August 1982,
Experimental eye research,
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
January 1973,
Biochimica et biophysica acta,
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
November 1983,
Investigative ophthalmology & visual science,
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
December 1978,
Experimental parasitology,
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
January 1977,
Comparative biochemistry and physiology. B, Comparative biochemistry,
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
May 1979,
Archives of andrology,
J Enouf, and
R Bredoux, and
B Boizard, and
J L Wautier, and
H Chap, and
J Thomas, and
M de Metz, and
S Levy-Toledano
April 1984,
Journal of bacteriology,
