Biomaterial Database

Identification of sequences involved in the transcriptional control of a Drosophila heat-shock gene. 1984

V Corces, and A Pellicer

We have used stable transformation of a cloned Drosophila hsp70 gene into mouse Ltk- cells as an assay system to determine which sequences are involved in the temperature-induced transcription of the gene. We have analyzed the effect of different external deletions on the ability of the gene to be transcribed after temperature elevation. We have also constructed chimeric genes containing different sequences of the hsp70 promoter region joined to the herpes simplex virus thymidine kinase gene, and we have studied the heat-shock inducibility of the thymidine kinase gene after transformation into mouse cells. Our results suggest that the heat-shock genes are under negative control and that their activation is the result of a derepression of the gene. In addition, our experiments indicate that the heat-shock consensus sequence is necessary but not sufficient for temperature-induced transcription, and its role seems to be functionally similar to other transcription signals (i.e. the CAT box) located upstream from the TATA box in several eukaryotic genes. We are proposing that there are two additional regulatory elements besides the consensus sequence which must be present for proper temperature-dependent transcription to occur. Either one of these elements, which are located on opposite sides of the TATA box, may serve at any one time to ensure transcription; that is, only one at a time is needed for proper expression of the heat-shock gene.

UI	MeSH Term	Description	Entries
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002678	Chimera	An individual that contains cell populations derived from different zygotes.	Hybrids,Chimeras,Hybrid
D002872	Chromosome Deletion	Actual loss of portion of a chromosome.	Monosomy, Partial,Partial Monosomy,Deletion, Chromosome,Deletions, Chromosome,Monosomies, Partial,Partial Monosomies
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004330	Drosophila	A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.	Fruit Fly, Drosophila,Drosophila Fruit Flies,Drosophila Fruit Fly,Drosophilas,Flies, Drosophila Fruit,Fly, Drosophila Fruit,Fruit Flies, Drosophila
D004720	Endonucleases	Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-.	Endonuclease
D005796	Genes	A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.	Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D006360	Heat-Shock Proteins	Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.	Stress Protein,Stress Proteins,Heat-Shock Protein,Heat Shock Protein,Heat Shock Proteins,Protein, Stress
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia