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In primary 2-day cultured bovine adrenocortical cells, adrenaline stimulated the steroidogenesis, while the effect of adrenaline did not appear in the freshly isolated cells. Thus the primary 2-day cultured cells were used to study the effect of adrenaline on steroidogenesis. Adrenaline showed the steroidogenesis-stimulating effect at concentrations higher than 10(-9) M, and the maximum effect was obtained between 10(-6) M and 10(-5) M in the primary 2-day cultured cells. The maximum effect of adrenaline was 50-70% of that of adrenocorticotropic hormone (ACTH). Noradrenaline, isoproterenol and phenylephrine also stimulated the steroidogenesis. However, the order of the potency was isoproterenol much greater than adrenaline = noradrenaline much much greater than phenylephrine. Propranolol and alprenolol inhibited the effect of adrenaline, but phenoxybenzamine and phentolamine did not inhibit the effect. Moreover, adrenaline stimulated the cyclic AMP production dose-dependently at concentrations higher than 10(-8) M. These results suggest that there are steroidogenesis-linked adrenergic receptors in primary 2-day cultured bovine adrenocortical cell membrane and that the steroidogenesis-stimulating effect of adrenaline occurs through the beta-adrenergic receptor.
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
October 2003,
Journal of pharmacological sciences,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
March 1992,
The Journal of endocrinology,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
January 1984,
Hormone research,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
February 1992,
Biochemical Society transactions,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
July 1983,
Endocrinology,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
August 1990,
Endocrinology,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
July 1979,
Endocrinology,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
July 1987,
Endocrinology,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
January 1982,
Molecular and cellular endocrinology,
M Kawamura, and
N Nakamichi, and
N Imagawa, and
Y Tanaka, and
C Tomita, and
M Matsuba
January 1990,
Japanese journal of pharmacology,
