-transparent.png){kind=logo}
Messenger RNA isolated from chicken pituitaries was used to construct a chicken pituitary cDNA library. A chicken growth hormone cDNA clone was isolated using 32P-labeled mammalian growth hormone cDNA probes. The amino acid sequence (derived from the DNA sequence) of the mature form of chicken growth hormone shows 77% homology with that of bovine growth hormone. The chicken growth hormone cDNA clone was used to generate a vector capable of producing chicken growth hormone in Escherichia coli. The recombinant E. coli-derived chicken growth hormone was similar to pituitary chicken growth hormone in several biochemical and immunological properties. The recombinant-derived hormone has been used to establish a sensitive radioimmunoassay for growth hormone determinations made from chicken sera. The chicken growth hormone gene has also been introduced into a retroviral vector capable of establishing productive infections of chicken cells both in in vitro and in vivo. The resulting infections are accompanied by the production of radioimmunoassay-detectable growth hormone. The concentrations of growth hormone in sera of Leghorn chickens infected with the recombinant retrovirus are three- to tenfold higher than in control animals.
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
April 1984,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
March 1986,
Lancet (London, England),
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
January 1988,
General and comparative endocrinology,
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
February 1990,
Journal of chromatography,
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
January 1993,
Problemy endokrinologii,
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
January 1988,
Growth, development, and aging : GDA,
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
January 1988,
Acta paediatrica Scandinavica. Supplement,
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
January 1986,
Reproduction, nutrition, developpement,
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
July 1990,
Journal of the Medical Association of Thailand = Chotmaihet thangphaet,
L M Souza, and
T C Boone, and
D Murdock, and
K Langley, and
J Wypych, and
D Fenton, and
S Johnson, and
P H Lai, and
R Everett, and
R Y Hsu
January 1990,
Revista do Hospital das Clinicas,
