Biomaterial Database

Nucleotide sequence of cloned DNA segments of the Haemophilus influenzae bacteriophage HP1c1. 1984

R C Benjamin, and W P Fitzmaurice, and P C Huang, and J J Scocca

Restriction fragments obtained by digestion of Haemophilus influenzae phage HP1c1 DNA with HaeIII have been cloned by insertion into the HindIII site of pBR322 using synthetic linkers. The nucleotide sequences have been determined for three adjacent fragments, HaeIII-E, HaeIII-C and HaeIII-K, which comprise and 8.2-kb segment of the HP1c1 genome. The distribution and location of restriction sites in the sequenced region were determined. Restriction sites containing the dinucleotides -GG- and -CC- occurred infrequently in the sequence. The region contains numerous sequences which are subsets of the high-affinity recognition sequence of Haemophilus transformation, including five sites which direct high-affinity uptake of fragments containing them. Shorter subsets of the uptake sequence, including those with little or no measurable affinity for the DNA transport system, are considerably over-represented in HP1c1 DNA. Nine open reading frames (ORFs) corresponding to presumed polypeptides longer than 90 amino acid residues were identified; all of these shared a common orientation, suggesting the probable direction of transcription in this segment of the phage genome. These ORFs were preceded by appropriately spaced polypurine stretches which might function as ribosome-binding sites. One ORF coincides with the site of a mutation affecting the production of phage tails.

UI	MeSH Term	Description	Entries
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004262	DNA Restriction Enzymes	Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.	Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D005814	Genes, Viral	The functional hereditary units of VIRUSES.	Viral Genes,Gene, Viral,Viral Gene
D006193	Haemophilus influenzae	A species of HAEMOPHILUS found on the mucous membranes of humans and a variety of animals. The species is further divided into biotypes I through VIII.	Bacterium influenzae,Coccobacillus pfeifferi,Haemophilus meningitidis,Hemophilus influenzae,Influenza-bacillus,Mycobacterium influenzae
D001435	Bacteriophages	Viruses whose hosts are bacterial cells.	Phages,Bacteriophage,Phage
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014764	Viral Proteins	Proteins found in any species of virus.	Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral