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Cellular retinol binding protein (CRBP) and cellular retinoic acid binding protein (CRABP) were assayed in bovine eye pigment epithelium (PE) cytosol from both early fetal and young adult animals. Both fetal and adult PE contain CRBP but only fetal PE contains CRABP. Blood retinol binding protein (RBP) was also assayed in serum from fetal and adult animals. Both fetal and adult serum contain RBP. A novel albumin adsorption technique (Affi-Gel Blue) combined with refinements of the sucrose gradient centrifugation technique provide a better assay method for retinoid receptors than polyacrylamide disc gel electrophoresis. Our findings support the hypothesis that retinoid receptors may cause or be the consequence of differentiation in animal tissues.
M E Cullum, and
B C Johnson, and
M H Zile
October 1976,
Biochimica et biophysica acta,
M E Cullum, and
B C Johnson, and
M H Zile
October 1979,
Federation proceedings,
M E Cullum, and
B C Johnson, and
M H Zile
May 1976,
Experimental eye research,
M E Cullum, and
B C Johnson, and
M H Zile
January 1980,
Methods in enzymology,
M E Cullum, and
B C Johnson, and
M H Zile
January 1979,
Current topics in eye research,
M E Cullum, and
B C Johnson, and
M H Zile
August 1977,
The Journal of biological chemistry,
M E Cullum, and
B C Johnson, and
M H Zile
January 1978,
World review of nutrition and dietetics,
M E Cullum, and
B C Johnson, and
M H Zile
January 1983,
Advances in nutritional research,
M E Cullum, and
B C Johnson, and
M H Zile
June 1977,
Nutrition reviews,
M E Cullum, and
B C Johnson, and
M H Zile
September 1978,
The Journal of biological chemistry,
