Biomaterial Database

Metal-ion mediated interaction of luciferase with tetraiodofluorescein. 1984

S Rajgopal, and M A Vijayalakshmi

Tetraiodofluorescein binds to luciferase in an apparently competitive manner, according to kinetic inactivation studies. Among the metal ions studied, Cu2+ and Ni2+ are the only two of the first row transition series that enhance enzyme inactivation by the dye. Mg2+, Co2+ and Mn2+ seem to have a protective effect whereas Zn2+ prevents enzyme inactivation and even enhances its activity. Luciferase bound to tetraiodofluorescien-Sepharose via a metal ion can subsequently be eluted with appropriate ligands. Luciferase was found to be sensitive to the spacer geometry of immobilized tetraiodofluorescein.

UI	MeSH Term	Description	Entries
D007313	Insecta	Members of the phylum ARTHROPODA composed or organisms characterized by division into three parts: head, thorax, and abdomen. They are the dominant group of animals on earth with several hundred thousand different kinds. Three orders, HEMIPTERA; DIPTERA; and SIPHONAPTERA; are of medical interest in that they cause disease in humans and animals. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p1).	Insects,Insect
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008156	Luciferases	Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.	Luciferase
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D002621	Chemistry	A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D002847	Chromatography, Agarose	A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.	Chromatography, Sepharose,Agarose Chromatography,Sepharose Chromatography,Agarose Chromatographies,Chromatographies, Agarose,Chromatographies, Sepharose,Sepharose Chromatographies
D004923	Erythrosine	A tetraiodofluorescein used as a red coloring in some foods (cherries, fish), as a disclosure of DENTAL PLAQUE, and as a stain of some cell types. It has structural similarity to THYROXINE.	FD & C Red No. 3,2',4',5',7'-Tetraiodofluorescein,Erythrosin,Erythrosin B,Erythrosine B,F D & C #3,FDC Red No. 3
D005452	Fluoresceins	A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.	Tetraiodofluorescein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor