Biomaterial Database

Solubilization of two molecular forms of the frog brain opioid receptor. 1984

A Puget, and B Frances, and P Jauzac, and J C Meunier

In equilibrium binding studies, using 3H-etorphine and 3H-diprenorphine, digitonin extracts of frog brain membranes are found to contain two classes of sites, one of which is seen only in the presence of Na+ ions. Centrifugation of the extracts in sucrose gradients separates two macromolecular components (10S and 12S) which display specific opiate binding activity. The 12S component appears to carry the site that binds opiates in the absence of Na+ ions while the 10S component would carry the other site, i.e. the one which is seen only in the presence of Na+ ions in equilibrium binding studies. Preliminary evidence is also given that in extracts of frog brain membranes which have been pre-incubated with 120 mM NaCl, the balance of the two components is shifted in favor of the slower sedimenting (10S) one. These results are discussed in terms of the regulation of the state of equilibrium between an agonist (12S) and an antagonist (10S) form of the opioid receptor.

UI	MeSH Term	Description	Entries
D011898	Ranidae	The family of true frogs of the order Anura. The family occurs worldwide except in Antarctica.	Frogs, True,Rana,Frog, True,True Frog,True Frogs
D011957	Receptors, Opioid	Cell membrane proteins that bind opioids and trigger intracellular changes which influence the behavior of cells. The endogenous ligands for opioid receptors in mammals include three families of peptides, the enkephalins, endorphins, and dynorphins. The receptor classes include mu, delta, and kappa receptors. Sigma receptors bind several psychoactive substances, including certain opioids, but their endogenous ligands are not known.	Endorphin Receptors,Enkephalin Receptors,Narcotic Receptors,Opioid Receptors,Receptors, Endorphin,Receptors, Enkephalin,Receptors, Narcotic,Receptors, Opiate,Endorphin Receptor,Enkephalin Receptor,Normorphine Receptors,Opiate Receptor,Opiate Receptors,Opioid Receptor,Receptors, Normorphine,Receptors, beta-Endorphin,beta-Endorphin Receptor,Receptor, Endorphin,Receptor, Enkephalin,Receptor, Opiate,Receptor, Opioid,Receptor, beta-Endorphin,Receptors, beta Endorphin,beta Endorphin Receptor,beta-Endorphin Receptors
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D004174	Diprenorphine	A narcotic antagonist similar in action to NALOXONE. It is used to remobilize animals after ETORPHINE neuroleptanalgesia and is considered a specific antagonist to etorphine.	Diprenorphine Hydrochloride,Revivon,Hydrochloride, Diprenorphine
D005048	Etorphine	A narcotic analgesic morphinan used as a sedative in veterinary practice.	Ethorphine,M99
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012964	Sodium	A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.	Sodium Ion Level,Sodium-23,Ion Level, Sodium,Level, Sodium Ion,Sodium 23
D012995	Solubility	The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Solubilities

Related Publications

A Puget, and B Frances, and P Jauzac, and J C Meunier

Solubilization of frog brain and retina cholinesterase and studies of different molecular forms.

January 1979, Comparative biochemistry and physiology. B, Comparative biochemistry,

A Puget, and B Frances, and P Jauzac, and J C Meunier

Characterization, solubilization and purification of an opioid receptor from the brain of the frog Rana ridibunda.

January 1986, NIDA research monograph,

A Puget, and B Frances, and P Jauzac, and J C Meunier

Solubilization and characterization of opioid binding sites from frog (Rana esculenta) brain.

October 1984, Journal of neurochemistry,

A Puget, and B Frances, and P Jauzac, and J C Meunier

Solubilization and purification of opioid receptor molecules of rat brain.

January 1989, Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao,

A Puget, and B Frances, and P Jauzac, and J C Meunier

[Solubilization and purification of opioid receptor molecules of rat brain].

April 1989, Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae,

A Puget, and B Frances, and P Jauzac, and J C Meunier

Distinct molecular forms of opiate binding in the frog brain.

January 1983, Life sciences,

A Puget, and B Frances, and P Jauzac, and J C Meunier

Separation of kappa-opioid receptor subtype from frog brain.

April 1985, FEBS letters,

A Puget, and B Frances, and P Jauzac, and J C Meunier

Solubilization of chicken brain cholinesterase, separation and characterization of molecular forms.

January 1978, General pharmacology,