-transparent.png){kind=logo}
A Sep-Pak C18 cartridge was used for purification of bile acids from serum. Three kinds of deuterium labeled internal standards were required for accurate measurement of individual sulfated and nonsulfated bile acids. These internal standards were added to the serum before its application to the cartridge. Separation of sulfated and nonsulfated bile acids was performed on piperidinohydroxypropyl Sephadex LH-20 column chromatography. The nonsulfate fraction was submitted to alkaline hydrolysis, and the sulfate fraction to solvolysis followed by alkaline hydrolysis. Each fraction was converted to the hexafluoroisopropyl-trifluoroacetyl derivatives and quantitated by mass fragmentography. The recovery of each bile acid sulfate was quite satisfactory. In fasting healthy subjects the mean of total nonsulfated bile acids in serum was 1.324 micrograms/ml, and that of total sulfated bile acids was 0.450 micrograms/ml. Sulfated lithocholic acid comprised a large part of sulfated bile acids in healthy subjects.
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
June 1981,
Journal of biochemistry,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
June 1983,
Gastroenterologia Japonica,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
March 1974,
Journal of lipid research,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
November 1972,
Lipids,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
March 1975,
Gastroenterology,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
January 1986,
Digestion,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
June 1988,
Acta paediatrica Japonica : Overseas edition,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
October 1982,
Journal of biochemistry,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
August 1978,
The Journal of laboratory and clinical medicine,
N Murata, and
T Beppu, and
H Takikawa, and
H Otsuka, and
T Kasama, and
Y Seyama
September 1984,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
