-transparent.png){kind=logo}
Autoradiographic localization of [3H]flunitrazepam in nuclei of the rat cerebral cortex was further confirmed by biochemical analysis of specific nuclear binding. Highly purified rat cerebral cortex nuclei were shown to bind [3H]flunitrazepam specifically. The Kd(app) for nuclear binding was 28 nM for the nuclei compared with a Kd(app) of 1.1 nM for binding of [3H] flunitrazepam to synaptosomal membrane fractions of the same tissue. Inhibition of the nuclear binding with inosine and hypoxanthine was greater than inhibition of the synaptic membrane fractions. These results lead to to conclude that specific binding may occur at both the synaptic membrane and the nuclear levels and that different endogenous ligands may compete at each site for binding. Furthermore, the possibility exists for translocation and alteration of the bound ligand complex from membrane site to nuclear site.
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
December 1982,
Journal of neurochemistry,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
August 1986,
The Journal of pharmacology and experimental therapeutics,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
December 1984,
Neuroscience letters,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
March 1979,
Cell biology international reports,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
April 1986,
Journal of neurochemistry,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
November 2001,
Analytical biochemistry,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
November 1992,
Brain research,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
January 1986,
General pharmacology,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
February 2008,
Progress in neuro-psychopharmacology & biological psychiatry,
H B Bosmann, and
D P Penney, and
K R Case, and
K Averill
January 1987,
Progress in neuro-psychopharmacology & biological psychiatry,
