BIOMAT Database Logo BIOMAT Database Logo

[Calorimetric assay of yeast inorganic pyrophosphatase interaction with magnesium and phosphate ions]. 1982

N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich

The thermodynamic characteristics for the specific binding of one or two Mg2+ by the yeast inorganic pyrophosphatase and for the enzyme interaction with phosphate were determined. Saturation of the first binding site with Mg2+ causes structural rearrangements in the enzyme molecule without changing the temperature of protein denaturation. On the contrast, saturation of the second binding site results in stabilization of the system, i. e. a considerable fall in the entropy and a rise in the temperature of denaturation. Phosphorylation of the enzyme carboxylic group by inorganic phosphate requires saturation of the first binding site with Mg2+ and is not accompanied by changes in the enthalpy of the system. The pyrophosphate synthesis in the presence of the enzyme saturated with Mg2+ in both binding sites is associated with changes in the enthalpy and, possibly, in the entropy of the system.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008274 Magnesium A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
D010710 Phosphates Inorganic salts of phosphoric acid. Inorganic Phosphate,Phosphates, Inorganic,Inorganic Phosphates,Orthophosphate,Phosphate,Phosphate, Inorganic
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D011755 Pyrophosphatases A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-. Pyrophosphatase
D002151 Calorimetry The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D043564 Inorganic Pyrophosphatase An enzyme which catalyzes the hydrolysis of diphosphate (DIPHOSPHATES) into inorganic phosphate. The hydrolysis of pyrophosphate is coupled to the transport of HYDROGEN IONS across a membrane. Pyrophosphatase, Inorganic,H(+)-PPase,H+-Pyrophosphatase,Proton-Pumping Inorganic Pyrophosphatase,Proton-Translocating Pyrophosphatase,Pyrophosphate-Energized Inorganic Pyrophosphatase,H+ Pyrophosphatase,Inorganic Pyrophosphatase, Proton-Pumping,Inorganic Pyrophosphatase, Pyrophosphate-Energized,Proton Pumping Inorganic Pyrophosphatase,Proton Translocating Pyrophosphatase,Pyrophosphatase, Proton-Pumping Inorganic,Pyrophosphatase, Proton-Translocating,Pyrophosphatase, Pyrophosphate-Energized Inorganic,Pyrophosphate Energized Inorganic Pyrophosphatase

Related Publications

N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
The interaction of uranyl ions with inorganic pyrophosphatase from baker's yeast.
January 1978, Acta biologica et medica Germanica,
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
Activation of yeast inorganic pyrophosphatase by magnesium.
October 1972, Biochemical and biophysical research communications,
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
[The role of magnesium ions in hydrolysis of pyrophosphate by yeast inorganic pyrophosphatase].
January 1973, Biokhimiia (Moscow, Russia),
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
[Effect of magnesium ions on the thermostability of inorganic pyrophosphatase from baker's yeast].
January 1979, Acta biologica et medica Germanica,
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
[Reaction of yeast inorganic pyrophosphatase with radioactive inorganic phosphate and pyrophosphate].
January 1973, Biokhimiia (Moscow, Russia),
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
Spectral and kinetic studies of phosphate and magnesium ion binding to yeast inorganic pyrophosphatase.
June 1989, European journal of biochemistry,
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
Kinetic studies of the interaction of uranyl ions with inorganic pyrophosphatase from baker's yeast.
January 1978, Acta biologica et medica Germanica,
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
Divalent metal ion, inorganic phosphate, and inorganic phosphate analogue binding to yeast inorganic pyrophosphatase.
October 1981, Biochemistry,
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
[Interaction of terbium ions with inorganic pyrophosphatase from bakers' yeast: characterization of the binding sites].
January 1982, Acta biologica et medica Germanica,
N N Vorob'eva, and T I Nazarova, and N P Bakuleva, and S M Avaeva, and I I Protasevich
[The interaction of yeast inorganic pyrophosphatase and carboxypeptidase A].
January 1970, Biokhimiia (Moscow, Russia),
Copied contents to your clipboard!