Biomaterial Database

Neuropeptide proctolin associated with an identified skeletal motoneuron. 1982

M O'Shea, and C A Bishop

Our objective was to find large and identifiable peptide-containing neurons and their postsynaptic targets. For this, a whole mount immunohistochemical method was used to locate cell bodies of neurons in the cockroach central nervous system immunoreactive to antibodies raised against the pentapeptide proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH). The morphology and projections of the immunoreactive neurons were investigated by combining intracellular dye injection and electrophysiological and immunochemical procedures. The presence of proctolin in specific immunoreactive neurons was check by high pressure liquid chromatography combined with bioassay applied to extracts made from individually identified and isolated cell bodies. Using these approaches, we have identified a large proctolin-containing motoneuron which innervates muscles in a proximal segment of the cockroach leg. This study establishes a well characterized cellular preparation in which the physiological actions and functions of a peptide-containing neuron can be studied in vivo.

UI	MeSH Term	Description	Entries
D009046	Motor Neurons	Neurons which activate MUSCLE CELLS.	Neurons, Motor,Alpha Motorneurons,Motoneurons,Motor Neurons, Alpha,Neurons, Alpha Motor,Alpha Motor Neuron,Alpha Motor Neurons,Alpha Motorneuron,Motoneuron,Motor Neuron,Motor Neuron, Alpha,Motorneuron, Alpha,Motorneurons, Alpha,Neuron, Alpha Motor,Neuron, Motor
D009479	Neuropeptides	Peptides released by NEURONS as intercellular messengers. Many neuropeptides are also hormones released by non-neuronal cells.	Neuropeptide
D009842	Oligopeptides	Peptides composed of between two and twelve amino acids.	Oligopeptide
D003058	Cockroaches	Insects of the order Dictyoptera comprising several families including Blaberidae, BLATTELLIDAE, Blattidae (containing the American cockroach PERIPLANETA americana), Cryptocercidae, and Polyphagidae.	Blaberidae,Blattaria,Blattidae,Blattodea,Cryptocercidae,Dictyoptera,Polyphagidae,Cockroach,Blattarias,Blattodeas,Cockroache,Cockroachs,Dictyopteras
D004594	Electrophysiology	The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005724	Ganglia	Clusters of multipolar neurons surrounded by a capsule of loosely organized CONNECTIVE TISSUE located outside the CENTRAL NERVOUS SYSTEM.
D006110	Grasshoppers	Plant-eating orthopterans having hindlegs adapted for jumping. There are two main families: Acrididae and Romaleidae. Some of the more common genera are: Melanoplus, the most common grasshopper; Conocephalus, the eastern meadow grasshopper; and Pterophylla, the true katydid.	Acrididae,Locusts,Romaleidae,Grasshopper,Locust
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D018377	Neurotransmitter Agents	Substances used for their pharmacological actions on any aspect of neurotransmitter systems. Neurotransmitter agents include agonists, antagonists, degradation inhibitors, uptake inhibitors, depleters, precursors, and modulators of receptor function.	Nerve Transmitter Substance,Neurohormone,Neurohumor,Neurotransmitter Agent,Nerve Transmitter Substances,Neurohormones,Neurohumors,Neuromodulator,Neuromodulators,Neuroregulator,Neuroregulators,Neurotransmitter,Neurotransmitters,Substances, Nerve Transmitter,Transmitter Substances, Nerve,Substance, Nerve Transmitter,Transmitter Substance, Nerve