Biomaterial Database

Serum and urogenital antibody responses to Escherichia coli pili in cystitis. 1982

P Rene, and M Dinolfo, and F J Silverblatt

Antipili antibody (APA) was quantitated by enzyme-linked immunosorbent assay in the serum, urine, and vaginal secretions of 12 women with cystitis due to Escherichia coli. Pili were purified from the strain infecting each patient. The geometric mean titers of APA in serum taken at the initial visit were: immunoglobulin G (IgG), 23.7 +/- 3.9; IgA, 4.1 +/- 4.0; and IgM, 4.4 +/- 5.7. No consistent change was observed between samples obtained at 3 and 6 weeks. APA in serum from 10 control patients was assayed against a pool of pili assembled from the 12 infecting isolates. The geometric mean titers of the control sera were: IgG, 8.4 +/- 3.2; IgA, 9.6 +/- 2.4; and IgM, 0. Antipili IgG and IgM were significantly greater in the infected than in the control sera (P less than 0.05). APA was not detected in the urine or vaginal secretions from any infected or control patient. Antigenic relationships between the 12 infecting strains were investigated by indirect immune electron microscopy. All but 1 of 12 infecting strains shared antigenic determinants. Two patients had a second E. coli infection during the study. The pili of the reinfecting isolates showed partial antigenic cross-reactivity with the pili of the initial infecting bacteria. These observations indicate that although cystitis stimulates the development of serum APAs, it fails to stimulate local APA and thus may not engender any immunological barrier to subsequent recolonization.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007075	Immunoglobulin M	A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin.	Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D010861	Fimbriae, Bacterial	Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).	Bacterial Fimbriae,Bacterial Pili,Common Fimbriae,Common Pili,Pili, Bacterial,Pili, Common,Bacterial Fimbria,Bacterial Pilus,Common Fimbria,Common Pilus,Fimbria, Bacterial,Pilus, Bacterial,Fimbria, Common,Fimbriae, Common,Pilus, Common
D003556	Cystitis	Inflammation of the URINARY BLADDER, either from bacterial or non-bacterial causes. Cystitis is usually associated with painful urination (dysuria), increased frequency, urgency, and suprapubic pain.	Cystitides
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D004927	Escherichia coli Infections	Infections with bacteria of the species ESCHERICHIA COLI.	E coli Infections,E. coli Infection,Infections, E coli,Infections, Escherichia coli,E coli Infection,E. coli Infections,Escherichia coli Infection,Infection, E coli,Infection, E. coli,Infection, Escherichia coli
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D000907	Antibodies, Bacterial	Immunoglobulins produced in a response to BACTERIAL ANTIGENS.	Bacterial Antibodies