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1. The effect of the time of preincubation with 3 mM EGTA on oxytocin-induced Ca-free contraction of rat uterus was investigated. It was found 10(-2) unit/ml oxytocin induced sustained Ca-free contraction only after preincubation for 50 min or more. 2. The tension developed showed a biphasic relation to the time of preincubation with a minimum at 25 min. 3. Sustained Ca-free contraction could be repeated with only slight reduction in magnitude. 4. Oxytocin-induced Ca-free contraction was relaxed by isoproterenol. The effect of isoproterenol was inhibited by 3 X 10(-4) M 1,1-diphenyl-3-piperazino-1-butanol hydrochloride.
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