Biomaterial Database

Cerianthin lectins: a new group of agglutinins from Cerianthus membranaceus (Singapore). 1982

O M Koch, and C K Lee, and G Uhlenbruck

Cerianthus membranaceus (Singapore) is a marine metazoon that belongs as a hexacorallion to the group of cnidaria. The saline extracts from the tentacles reveal high titers of agglutination potency on neuraminidase-treated O-erythrocytes. With the help of affinity chromatography, more than 7 proteins with lectin activity which could all be inhibited by D-galactose were isolated. The lectins were separated on DEAE-sepharose, and the main component was purified after an additional step of gel filtration on Bio-Gel P 60. This main component is a non-glycosylated protein with a molecular weight of 38,400 ds consisting of a single protein chain and characterized by the lack of polymers and intermonomeric disulfide bonds. By electrofocusing, the pure main lectin shows two bands at pH 4.17 and pH 4.24. Optimal inhibition of the pure lectin is achieved by D-galactose containing oligo- and polysaccharides.

UI	MeSH Term	Description	Entries
D007526	Isoelectric Point	The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.	Isoelectric Points,Point, Isoelectric,Points, Isoelectric
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D002846	Chromatography, Affinity	A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D003063	Cnidaria	A phylum of radially symmetrical invertebrates characterized by possession of stinging cells called nematocysts. It includes the classes ANTHOZOA; CUBOZOA; HYDROZOA, and SCYPHOZOA. Members carry CNIDARIAN VENOMS.	Cnidarians,Cnidarian,Cnidarias
D005697	Galactosides	Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of galactose with an alcohol to form an acetal. They include both alpha- and beta-galactosides.
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D037102	Lectins	Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.	Animal Lectin,Animal Lectins,Isolectins,Lectin,Isolectin,Lectin, Animal,Lectins, Animal