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Theophylline is often utilized as a secretagogue in studies on the in vitro release of somatostatin and other hormones. We report here on the effect of theophylline on ligand binding in somatostatin radioimmunoassays performed with three different antisera. For all three radioimmunoassays, tracer binding both in the presence and absence of unlabelled somatostatin was inhibited by the addition of theophylline to the RIA reaction mixture. This effect occurred at final assay concentrations of theophylline which may likely be encountered when assaying samples obtained in studies of theophylline-induced hormone release in vitro. The possible interfering effect of theophylline in radioimmunoassays should be considered whenever theophylline is used as a tool to study hormone release.
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
January 1987,
Annals of internal medicine,
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
March 1987,
Lancet (London, England),
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
November 1980,
Clinical chemistry,
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
October 1980,
Clinical chemistry,
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
February 2024,
Journal of chemical information and modeling,
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
May 1982,
Clinical chemistry,
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
April 2009,
Journal of the American Chemical Society,
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
April 2022,
eLife,
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
November 1986,
Neuroscience,
W I Sivitz, and
B S Chertow, and
N G Baranetsky, and
S E Fix
March 2002,
Proceedings of the National Academy of Sciences of the United States of America,
