Biomaterial Database

Electron microscopic studies on multinucleate foreign body giant cells derived from Kupffer cells in mice given Indian ink intravenously. 1983

H Fujita, and S Kawamata, and K Yamashita

Changes in Kupffer cells in mice given intravenous Indian ink were studied with the electron microscope. Kupffer cells as well as hepatic sinusoidal endothelial cells take up Indian ink particles by pinocytosis. In addition, a few Ito cells and hepatocytes ingest a small number of particles. The carbon particles are stored in cytoplasmic vacuoles in all these cell types. Aggregates of Kupffer cells containing many vacuoles stuffed with carbon particles are seen in the sinusoidal lumen, Disse space and interlobular connective tissue space 3 and 4 days after injection with Indian ink. One month after the injection, large clumps of aggregated Kupffer cells containing numerous carbon-filled vacuoles are distributed in the Disse space and other connective tissue spaces. The Kupffer cells in these clumps are in close contact and are partly fused with one another. After 3, 4 and 6 months, large multinucleate foreign body giant cells with numerous large vacuoles containing densely-packed ink particles are seen throughout the liver tissue. They are considered to be formed by fusion of Kupffer cells. Some endothelial cells also store ink particles in cytoplasmic vacuoles for as long as 6 months after injection.

UI	MeSH Term	Description	Entries
D007275	Injections, Intravenous	Injections made into a vein for therapeutic or experimental purposes.	Intravenous Injections,Injection, Intravenous,Intravenous Injection
D007281	Ink	Pigmented or colored fluid used for writing, staining, painting, drawing, printing, or copying images.	Inks
D007728	Kupffer Cells	Specialized phagocytic cells of the MONONUCLEAR PHAGOCYTE SYSTEM found on the luminal surface of the hepatic sinusoids. They filter bacteria and small foreign proteins out of the blood and dispose of worn out red blood cells.	Kupffer Cell,Cell, Kupffer,Cells, Kupffer
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D008815	Mice, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.	Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D010873	Pinocytosis	The engulfing of liquids by cells by a process of invagination and closure of the cell membrane to form fluid-filled vacuoles.	Pinocytoses
D002450	Cell Communication	Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.	Cell Interaction,Cell-to-Cell Interaction,Cell Communications,Cell Interactions,Cell to Cell Interaction,Cell-to-Cell Interactions,Communication, Cell,Communications, Cell,Interaction, Cell,Interaction, Cell-to-Cell,Interactions, Cell,Interactions, Cell-to-Cell
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D004727	Endothelium	A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.	Endotheliums