Biomaterial Database

The interaction of thyrotrophin binding inhibiting immunoglobulins with high and low affinity TSH receptors. 1983

F Pekonen, and T Mäkinen

The role of high and low affinity TSH receptors in thyrotrophin binding inhibiting immunoglobulin (TBII) measurements was evaluated. By modifying binding conditions it was possible to observe separately mainly high or low affinity TSH receptors. The greatest difference between TBII positive and negative immunoglobulins was observed when binding to high affinity receptors was measured. In contrast the difference was almost completely lost when low affinity receptors were exposed. The TBII and TSH sensitivity of the radioreceptor assay did however not completely correlate, thus suggesting a difference between TBII and TSH binding sites. During normal pregnancy a high ratio of borderline positive TBII titres was observed in a routine TBII radioreceptor binding assay. Such an assay measures concomitantly both high and low affinity receptors. By using binding conditions measuring preferentially high affinity TSH receptors the TBII positivity in normal pregnancy greatly decreased from 71 to 13%. These results suggest that TBII assay sensitivity and specificity are greatly improved when high affinity TSH receptors are observed. This is especially of importance during pregnancy when false positive TBII indexes are often seen.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011869	Radioligand Assay	Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).	Protein-Binding Radioassay,Radioreceptor Assay,Assay, Radioligand,Assay, Radioreceptor,Assays, Radioligand,Assays, Radioreceptor,Protein Binding Radioassay,Protein-Binding Radioassays,Radioassay, Protein-Binding,Radioassays, Protein-Binding,Radioligand Assays,Radioreceptor Assays
D011956	Receptors, Cell Surface	Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.	Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D011989	Receptors, Thyrotropin	Cell surface proteins that bind pituitary THYROTROPIN (also named thyroid stimulating hormone or TSH) and trigger intracellular changes of the target cells. TSH receptors are present in the nervous system and on target cells in the thyroid gland. Autoantibodies to TSH receptors are implicated in thyroid diseases such as GRAVES DISEASE and Hashimoto disease (THYROIDITIS, AUTOIMMUNE).	Receptors, Thyroid Stimulating Hormone,TSH Receptors,Thyroid Stimulating Hormone Receptors,Thyrotropin Receptors,LATS Receptors,Receptor, LATS Immunoglobulins,Receptors, LATS,Receptors, Long-Acting Thyroid Stimulator,Receptors, TSH,TSH Receptor,Thyroid Stimulating Hormone Receptor,Thyrotropin Receptor,Receptor, TSH,Receptor, Thyrotropin,Receptors, Long Acting Thyroid Stimulator
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D018828	Immunoglobulins, Thyroid-Stimulating	Autoantibodies that bind to the thyroid-stimulating hormone (TSH) receptor (RECEPTORS, THYROTROPIN) on thyroid epithelial cells. The autoantibodies mimic TSH causing an unregulated production of thyroid hormones characteristic of GRAVES DISEASE.	Antibodies, Thyroid-Stimulating,Ig Thyroid Stimulating,Thyroid-Stimulating Antibodies,Thyroid-Stimulating Autoantibodies,Thyroid-Stimulating Autoantibody,Thyroid-Stimulating Immunoglobulins,Immunoglobulin, Thyroid-Stimulating,Thyroid Stimulating Antibody,Thyroid-Stimulating Immunoglobulin,Antibody, Thyroid Stimulating,Autoantibody, Thyroid-Stimulating,Immunoglobulin, Thyroid Stimulating,Stimulating Antibody, Thyroid,Thyroid Stimulating Antibodies,Thyroid Stimulating Autoantibodies,Thyroid Stimulating Autoantibody,Thyroid Stimulating Immunoglobulin,Thyroid Stimulating Immunoglobulins,Thyroid Stimulating, Ig