Biomaterial Database

Electron microscopic studies on the structure of 60-70S RNA of avian myeloblastosis virus. 1982

J Stokrová, and J Korb, and J Ríman

Structural properties of the 60-70S RNA complex of avian myeloblastosis virus (AMV) were analysed in electron microscope after treatment under a set of non-denaturing, gently and strongly denaturing conditions. By selected denaturing conditions, the significant fraction of 60-70S AMV RNA molecules revealed partially unfolded structures either in a dimer or a more complex form and in a length corresponding to mol. wt. of 5.6 X 10(6). The typical dimers contained a characteristic central structure connecting the subunits and similar to those described for Rous sarcoma virus (RSV) and mammalian retrovirus RNAs. This dimer linkage in the AMV genome occurred at 384 +/- 43 nucleotides from one end of each subunit. Besides partially unfolded complexes, collapsed structures and extended linear molecules were observed. The length of majority of the linear molecules had reached a half of that of the partially unfolded complexes corresponding to the mol. wt. of monomers estimated under conditions of strong denaturation to be 2.8 X 10(6). Based on our findings, we conclude that the genome of AMV shares the dimer structure with RSV and mammalian retroviruses. We also conclude that the secondary structure of AMV RNA molecule is more labile than that of RNA of mammalian retroviruses.

UI	MeSH Term	Description	Entries
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D009189	Avian Myeloblastosis Virus	A species of ALPHARETROVIRUS causing anemia in fowl.	Myeloblastosis Virus, Avian,Avian Myeloblastosis Viruses,Myeloblastosis Viruses, Avian,Virus, Avian Myeloblastosis,Viruses, Avian Myeloblastosis
D009690	Nucleic Acid Conformation	The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.	DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D009691	Nucleic Acid Denaturation	Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.	DNA Denaturation,DNA Melting,RNA Denaturation,Acid Denaturation, Nucleic,Denaturation, DNA,Denaturation, Nucleic Acid,Denaturation, RNA,Nucleic Acid Denaturations
D005559	Formamides	A group of amides with the general formula of R-CONH2.
D001354	Avian Leukosis Virus	The type species of ALPHARETROVIRUS producing latent or manifest lymphoid leukosis in fowl.	Leukosis Virus, Avian,Lymphomatosis Virus, Avian,Rous-Associated Virus,Avian Leukosis Viruses,Avian Lymphomatosis Virus,Avian Lymphomatosis Viruses,Leukosis Viruses, Avian,Lymphomatosis Viruses, Avian,Rous Associated Virus,Virus, Avian Leukosis,Virus, Avian Lymphomatosis,Virus, Rous-Associated,Viruses, Avian Leukosis,Viruses, Avian Lymphomatosis
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D014508	Urea	A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.	Basodexan,Carbamide,Carmol
D046911	Macromolecular Substances	Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.	Macromolecular Complexes,Macromolecular Compounds,Macromolecular Compounds and Complexes,Complexes, Macromolecular,Compounds, Macromolecular,Substances, Macromolecular