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Experiments were performed in order to determine the minimal requirement for the proteins L7/L12 in polyphenylalanine synthesis and elongation factor EF-G-dependent GTP hydrolysis. Via reconstitution, ribosomal particles were prepared containing variable amounts of L7/L12. The L7/L12 content of these particles was carefully determined by the use of 3H-labelled L7/L12 and by radioimmunoassay. The activity of the particles was determined as a function of the L7/L12 content. Our results show that only one dimer of L7/L12 is required for full activity in EF-G-dependent GTP hydrolysis. On the other hand, two L7/L12 dimers are required for polyphenylalanine synthesis. In addition, we have determined the relation between the number of L7/L12 stalks, as observed by electron microscopy, and the L7/L12 content of the 50 S particles. Our interpretation of these results is that each ribosomal particle possesses two L7/L12 binding sites, each site being involved in binding one dimer. Binding of L7/L12 dimer in one site gives rise to formation of the L7/L12 stalk, whereas binding in the other site has no effect on the number of visible stalks.
W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
October 1976,
European journal of biochemistry,
W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
November 1976,
FEBS letters,
W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
January 1977,
FEBS letters,
W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
December 1974,
Proceedings of the National Academy of Sciences of the United States of America,
W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
December 1996,
Biochemistry,
W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
August 1981,
Biochemistry,
W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
June 1989,
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W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
November 1978,
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W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
October 1981,
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W Möller, and
P I Schrier, and
J A Maassen, and
A Zantema, and
E Schop, and
H Reinalda, and
A F Cremers, and
J E Mellema
October 1989,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
