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Friend erythroleukemia cells (FL cells) derived from DBA/2 mice may be induced to differentiate in vitro by addition of dimethyl sulfoxide (DMSO) to the culture medium. Transglutaminase (glutaminyl-peptide gamma-glutamyltransferase, EC 2.3.2.13) (TGase) activity was detected in the lysates of uninduced FL cells and was markedly increased in DMSO-treated cells. DMSO induced TGase activity of differentiating FL cells in a dose-dependent manner over the concentration range 0-280 mM. The increase in TGase activity was observed after 1 day's cultivation of the cells with 280 mM DMSO and continued to 4 days. Another differentiation inducing agent, butyric acid (1.4 mM), was as effective as DMSO in enhancing TGase activity in FL cells. Treatment of controls, a lymphoma cell line and mouse erythrocytes, with DMSO or butyric acid had no effect. These results suggest that the increase in TGase activity in DMSO- or butyric acid-treated FL cells may be related to cell differentiation.
K H Hsu, and
H Friedman
October 1983,
Proceedings of the National Academy of Sciences of the United States of America,
K H Hsu, and
H Friedman
July 1984,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
K H Hsu, and
H Friedman
October 1971,
Cancer research,
K H Hsu, and
H Friedman
April 1985,
The Journal of biological chemistry,
K H Hsu, and
H Friedman
October 1979,
Cancer research,
K H Hsu, and
H Friedman
December 1986,
Cancer research,
K H Hsu, and
H Friedman
June 1982,
FEBS letters,
K H Hsu, and
H Friedman
March 1984,
Biochemical and biophysical research communications,
K H Hsu, and
H Friedman
September 1987,
Proceedings of the National Academy of Sciences of the United States of America,
K H Hsu, and
H Friedman
January 1985,
Experimental cell biology,
