-transparent.png){kind=logo}
To enable in vitro characterization of nortriptyline 10-hydroxylase activity of microsomal fractions of human liver, methods for the determination of E- and Z-10-hydroxynortriptyline were developed. High performance liquid chromatography (HPLC) with UV-detection or HPLC separation followed by quantitation by gas chromatography-mass spectrometry were used. The microsomal 10-hydroxylation of nortriptyline was linear to 60 min with a protein concentration of 1 mg x ml-1. In 15 human livers a 5-fold variation in activity was found. The formation of the Z-isomer comprised 13 +/- 3% (mean +/- SD) of the total 10-hydroxylase activity. The 10-hydroxylation was inhibited by debrisoquine, sparteine, propranolol, and encainide. No effect or a stimulation of the activity was seen with p-nitroanisole and acetanilide.
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
January 1981,
Drug metabolism and disposition: the biological fate of chemicals,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
May 1991,
Therapeutic drug monitoring,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
January 1989,
Therapeutic drug monitoring,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
December 2008,
Xenobiotica; the fate of foreign compounds in biological systems,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
February 1991,
The Journal of biological chemistry,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
July 2005,
Molecular genetics and metabolism,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
January 1989,
Drug metabolism and disposition: the biological fate of chemicals,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
April 1986,
Acta pharmacologica et toxicologica,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
March 2000,
Drug metabolism and disposition: the biological fate of chemicals,
B Mellström, and
L Bertilsson, and
C Birgersson, and
M Göransson, and
C von Bahr
August 1983,
Life sciences,
