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The uropathogenic strain Escherichia coli J96 mediates mannose-resistant hemagglutination owing to production of a digalactoside-binding adhesin. A cosmid clone from this strain has been isolated that, when harbored in E. coli K-12, expressed Pap pili and this adhesin (R. Hull et al., Infect. Immun. 33:933-938, 1981). By transposon mutagenesis and by the construction of a number of hybrid plasmid derivatives, we have demonstrated that about 8.5 kilobases of DNA is required to generate a mannose-resistant hemagglutination-positive phenotype in E. coli K-12 strain P678-54. The structural gene for the Pap pili monomer, papA, has been identified and mapped close to the promotor-proximal end of the Pap operon. Although strain P678-54 that harbored a Tn5 insertion within papA showed a mannose-resistant hemagglutination-positive phenotype, it was negative in a competitive enzyme-linked immunosorbent assay with anti-Pap pilus serum. This could mean that a Pap adhesin is encoded by a region on the Pap operon that is distinct from papA.
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
June 1991,
Infection and immunity,
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
January 1985,
Current topics in microbiology and immunology,
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
March 1989,
Molecular microbiology,
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
May 2003,
Bioorganic & medicinal chemistry,
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
January 2000,
Advances in experimental medicine and biology,
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
November 1998,
Molecular genetics and metabolism,
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
February 2008,
Infection and immunity,
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
March 1985,
Proceedings of the National Academy of Sciences of the United States of America,
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
August 1999,
Science (New York, N.Y.),
S Normark, and
D Lark, and
R Hull, and
M Norgren, and
M Båga, and
P O'Hanley, and
G Schoolnik, and
S Falkow
June 1985,
Journal of bacteriology,
