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An improved method for the solubilization of the alpha 1-adrenergic receptors in rat liver, utilizing digitonin, glycerol and sonication, is described. The yield of solubilized receptors was approximately 20%. The soluble receptors showed characteristics similar to the membrane-bound alpha 1 receptors. However, upon solubilization, the affinity for the agonists (-)norepinephrine and (-)epinephrine increased 35- to 66-fold when compared to the affinity in the membranes. The affinity for antagonists remained unchanged. A number of synthetic partial agonists showed a less marked (5- to 10-fold) increase in affinity upon solubilization. These data are consistent with the notion that these receptors might be capable of existing in two distinct conformational states with the high affinity state for agonists being favored by solubilization.
J E Wikberg, and
R J Lefkowitz, and
M G Caron
March 1978,
Molecular pharmacology,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
July 1979,
Molecular pharmacology,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
January 1981,
Advances in cyclic nucleotide research,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
July 1980,
Nature,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
August 1985,
FEBS letters,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
April 1982,
Proceedings of the National Academy of Sciences of the United States of America,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
April 1982,
FEBS letters,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
July 1981,
Proceedings of the National Academy of Sciences of the United States of America,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
August 1980,
Proceedings of the National Academy of Sciences of the United States of America,
J E Wikberg, and
R J Lefkowitz, and
M G Caron
May 1987,
Biochimica et biophysica acta,
