BIOMAT Database Logo BIOMAT Database Logo

Simple procedure for preparation of bluetongue virus and epizootic hemorrhagic disease virus antigens for agar gel immunodiffusion. 1983

J L Stott, and B I Osburn

A simplified procedure was developed for preparing soluble antigen from two related orbiviruses, bluetongue and epizootic hemorrhagic disease viruses, for agar gel immunodiffusion. The antigens gave excellent results in both micro-agar gel diffusion (agar gel precipitin) and macro-agar gel diffusion (bluetongue immunodiffusion). Minor modification in the spatial arrangement of reference antisera, commonly utilized in the agar gel immunodiffusion tests, was employed to reduce the possible development of false-positive reactions. The procedures for antigen preparation were inexpensive and did not require elaborate filtration or high-speed centrifugation. Stability of antigen preparations at 5 degrees C was excellent (in excess of 3 years for bluetongue virus and 2 years for epizootic hemorrhagic disease virus).

UI MeSH Term Description Entries
D012087 Reoviridae A family of unenveloped RNA viruses with cubic symmetry. The twelve genera include ORTHOREOVIRUS; ORBIVIRUS; COLTIVIRUS; ROTAVIRUS; Aquareovirus, Cypovirus, Phytoreovirus, Fijivirus, Seadornavirus, Idnoreovirus, Mycoreovirus, and Oryzavirus. Aquareovirus,Cypovirus,Cytoplasmic Polyhedrosis Viruses,Fijivirus,Idnoreovirus,Mycoreovirus,Oryzavirus,Phytoreovirus,Reoviruses, Aquatic,Respiratory Enteric Orphan Viruses,Seadornavirus,Aquareoviruses,Aquatic Reovirus,Aquatic Reoviruses,Cypoviruses,Cytoplasmic Polyhedrosis Virus,Fijiviruses,Idnoreoviruses,Mycoreoviruses,Oryzaviruses,Phytoreoviruses,Polyhedrosis Virus, Cytoplasmic,Polyhedrosis Viruses, Cytoplasmic,Reovirus, Aquatic,Seadornaviruses
D001819 Bluetongue A reovirus infection, chiefly of sheep, characterized by a swollen blue tongue, catarrhal inflammation of upper respiratory and gastrointestinal tracts, and often by inflammation of sensitive laminae of the feet and coronet. Blue Tongue,Tongue, Blue
D001820 Bluetongue virus The type species of ORBIVIRUS causing a serious disease in sheep, especially lambs. It may also infect wild ruminants and other domestic animals. Ovine Catarrhal Fever Virus,Blue Tongue Virus,Blue Tongue Viruses,Bluetongue Viruses
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002418 Cattle Diseases Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus. Bovine Diseases,Bovine Disease,Cattle Disease,Disease, Bovine,Disease, Cattle,Diseases, Bovine,Diseases, Cattle
D005779 Immunodiffusion Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction. Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006476 Orthohantavirus A genus of the family BUNYAVIRIDAE causing HANTAVIRUS INFECTIONS, first identified during the Korean war. Infection is found primarily in rodents and humans. Transmission does not appear to involve arthropods. HANTAAN VIRUS is the type species. Dobrava-Belgrade Virus,Hantavirus,Andes Hantavirus,Andes Virus,Andes Hantaviruses,Dobrava Belgrade Virus,Hantavirus, Andes,Hantaviruses,Hantaviruses, Andes,Orthohantaviruses
D006480 Hemorrhagic Fever with Renal Syndrome An acute febrile disease occurring predominately in Asia. It is characterized by fever, prostration, vomiting, hemorrhagic phenonema, shock, and renal failure. It is caused by any one of several closely related species of the genus ORTHOHANTAVIRUS. The most severe form is caused by HANTAAN VIRUS whose natural host is the rodent Apodemus agrarius. Milder forms are caused by SEOUL VIRUS and transmitted by the rodents Rattus rattus and R. norvegicus, and the PUUMALA VIRUS with transmission by Clethrionomys galreolus. HFRS,Hemorrhagic Fever, Epidemic,Hemorrhagic Fever, Korean,Hemorrhagic Nephroso-Nephritis,Nephropathia Epidemica,Epidemic Hemorrhagic Fever,Epidemic Hemorrhagic Fevers,Hemorrhagic Fevers, Epidemic,Hemorrhagic Nephroso Nephritis,Hemorrhagic Nephroso-Nephritides,Korean Hemorrhagic Fever,Nephroso-Nephritides, Hemorrhagic,Nephroso-Nephritis, Hemorrhagic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000956 Antigens, Viral Substances elaborated by viruses that have antigenic activity. Viral Antigen,Viral Antigens,Antigen, Viral

Related Publications

J L Stott, and B I Osburn
Comparison of indirect immunofluorescence with agar gel immunodiffusion for the diagnosis of bluetongue virus infection.
January 1992, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
J L Stott, and B I Osburn
Precipitating antigens of hemagglutinating encephalomyelitis virus demonstrated by agar gel immunodiffusion.
July 1972, American journal of veterinary research,
J L Stott, and B I Osburn
Agar-gel immunodiffusion assay for pseudorabies virus antibody.
May 1978, Journal of clinical microbiology,
J L Stott, and B I Osburn
Plaque neutralization of bluetongue virus and epizootic hemorrhagic disease virus in BHK21 cells.
November 1976, American journal of veterinary research,
J L Stott, and B I Osburn
Molecular epidemiology of two US orbiviruses: bluetongue virus and epizootic hemorrhagic disease virus.
January 1985, Progress in clinical and biological research,
J L Stott, and B I Osburn
Antigen capture competitive enzyme-linked immunosorbent assays using baculovirus-expressed antigens for diagnosis of bluetongue virus and epizootic hemorrhagic disease virus.
February 2004, Journal of clinical microbiology,
J L Stott, and B I Osburn
[Simple preliminary analysis of antigens and antibodies with unknown reaction relationships using agar gel immunodiffusion].
January 1970, Giornale di batteriologia, virologia, ed immunologia ed annali dell'Ospedale Maria Vittoria di Torino,
J L Stott, and B I Osburn
Prospective Study of Epizootic Hemorrhagic Disease Virus and Bluetongue Virus Transmission in Captive Ruminants.
July 2020, Journal of medical entomology,
J L Stott, and B I Osburn
Bluetongue virus and epizootic hemorrhagic disease virus survey in cattle of the Galapagos Islands.
March 2019, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
J L Stott, and B I Osburn
Rapid detection of bovid herpes virus 1 antigens by counter immunoelectrophoresis and agar-gel immunodiffusion.
February 1989, Tropical animal health and production,
Copied contents to your clipboard!