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Neutrophil homogenates contained a high affinity guanosine triphosphatase (GTPase) that was stimulatable (+27%) by the addition of 100 nM N-formyl chemotactic peptide (CHO-pep), but not by 1 microgram X ml-1 phorbolmyristate acetate (PMA). Kinetic analysis of the stimulation demonstrated an apparent lagtime of 14.3 +/- 6.9 s between the addition of CHO-pep and the optimal GTPase stimulation. The GTPase activity (but not CHO-pep-stimulated GTPase activity) was preserved in a highly purified plasma membrane fraction of the homogenate. From these observations we suggest that both a high affinity guanine nucleotide binding protein and GTPase are closely associated with the plasma membrane CHO-pep receptor. The possibility that GTPase activity may influence guanine nucleotide regulation of adenylate cyclase during CHO-pep stimulation of neutrophils is discussed.
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
January 1982,
Journal of cellular biochemistry,
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
November 1989,
Biochemical and biophysical research communications,
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
April 1981,
Journal of immunology (Baltimore, Md. : 1950),
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
November 1987,
Il Farmaco; edizione scientifica,
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
May 1990,
Journal of leukocyte biology,
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
February 1993,
Biochemistry,
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
June 1983,
The Journal of biological chemistry,
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
December 1990,
Biochemical and biophysical research communications,
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
September 1989,
FEBS letters,
P A Hyslop, and
Z G Oades, and
A J Jesaitis, and
R G Painter, and
C G Cochrane, and
L A Sklar
October 1986,
The Journal of clinical endocrinology and metabolism,
