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Precursor forms of lactase-phlorizin hydrolase, sucrase-isomaltase and aminopeptidase N were studied by pulse-labelling of organ-cultured human intestinal biopsies. After labelling the biopsies were fractionated by the Ca2+-precipitation method and the enzymes isolated by immunoprecipitation. The results indicate that the lactase-phlorizin hydrolase is synthesized as a Mr 245 000 polypeptide, which is intracellularly cleaved into its mature Mr 160 000 form. Sucrase-isomaltase is shown to be synthesized as a single chain precursor (Mr 245 000 and 265 000) while the precursor of aminopeptidase N is shown to be of apparently the same size as the mature enzyme (Mr 140 000 and 160 000).
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
October 1992,
Journal of pediatric gastroenterology and nutrition,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
July 1991,
Gene,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
December 1987,
The Journal of biological chemistry,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
November 1995,
Archives of biochemistry and biophysics,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
August 1995,
The Journal of biological chemistry,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
May 1974,
Biochimica et biophysica acta,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
July 1993,
Gastroenterology,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
April 2001,
Histology and histopathology,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
January 1990,
Biochemistry,
H Skovbjerg, and
E M Danielsen, and
O Noren, and
H Sjöström
January 1987,
The Biochemical journal,
