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Glutamate dehydrogenase activity was determined in mitochondrial preparations from rat ventral prostate and rat kidney. Kinetic parameters of the ventral prostate enzyme were comparable to those for the kidney enzyme. Glutamate dehydrogenase activity in the direction of glutamate oxidative deamination was inhibited by alpha-ketoglutarate. However, the characteristics of alpha-ketoglutarate inhibition indicated that glutamate oxidation via glutamate dehydrogenase can occur at in vivo prostatic alpha-ketoglutarate levels. These results suggest that glutamate dehydrogenase activity in prostate may provide a continuous source of alpha-ketoglutarate for aspartate transamination to oxalacetate and ultimate citrate synthesis. In addition prostate mitochondria are able to couple the glutamic dehydrogenase reaction to aspartate aminotransferase. Under these conditions aspartate in the presence of glutamate and acetyl coenzyme A will result in a net synthesis of citrate. Consequently we propose an aspartate-glutamate pathway for citrate synthesis in prostate.
R B Franklin, and
L C Costello
January 1986,
Acta biochimica Polonica,
R B Franklin, and
L C Costello
March 1982,
Archives of andrology,
R B Franklin, and
L C Costello
June 1976,
The Journal of endocrinology,
R B Franklin, and
L C Costello
August 1997,
Analytical biochemistry,
R B Franklin, and
L C Costello
January 1977,
Comparative biochemistry and physiology. B, Comparative biochemistry,
R B Franklin, and
L C Costello
November 2003,
The Biochemical journal,
R B Franklin, and
L C Costello
January 1981,
Progress in clinical and biological research,
R B Franklin, and
L C Costello
January 1989,
Radiobiologiia,
R B Franklin, and
L C Costello
June 1981,
Archives of biochemistry and biophysics,
R B Franklin, and
L C Costello
June 1974,
Endocrinologia japonica,
