Biomaterial Database

Sperm antibodies in infertile women: a comparison of three methods for handling the cervical mucus for extraction of immunoglobulins. 1984

M Cerasaro, and A Lenzi, and A Massacesi, and F Dondero

Three procedures commonly employed in the extraction of soluble proteins from cervical mucus, for the application of the technique for the detection of sperm antibodies also in this biological fluid, were compared in order to establish which provided the highest yield. Studies were therefore carried out on 125 women with primary and secondary sterility characterized by good rheologic qualities of their mid-cycle cervical mucus and in presence of repeatedly poor PCTs, not justified by the seminal characteristics of their partners. The laboratory results and the statistical analysis have shown that as far as concerns IgG, liquefaction with bromelin and solubilization with Baker's buffer provide a better yield than ultracentrifugation; while bromelin alone has the best yield with IgA.

UI	MeSH Term	Description	Entries
D007136	Immunoglobulins	Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.	Globulins, Immune,Immune Globulin,Immune Globulins,Immunoglobulin,Globulin, Immune
D007247	Infertility, Female	Diminished or absent ability of a female to achieve conception.	Sterility, Female,Sterility, Postpartum,Sub-Fertility, Female,Subfertility, Female,Female Infertility,Female Sterility,Female Sub-Fertility,Female Subfertility,Postpartum Sterility,Sub Fertility, Female
D008297	Male		Males
D001963	Bromelains	Protein-digesting and milk-clotting enzymes found in PINEAPPLE fruit juice and stem tissue. Enzymes from the two sources are distinguished as fruit bromelain and stem bromelain. This enzyme was formerly listed as EC 3.4.22.4.	Bromelins,Ananase,Bromelain,Bromelain-POS,Bromelin,Dayto Anase,Debrase,Dontisanin,Extranase,Mucozym,Proteozym,Traumanase,Bromelain POS,BromelainPOS
D002021	Buffers	A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.	Buffer
D002582	Cervix Mucus	A slightly alkaline secretion of the endocervical glands. The consistency and amount are dependent on the physiological hormone changes in the menstrual cycle. It contains the glycoprotein mucin, amino acids, sugar, enzymes, and electrolytes, with a water content up to 90%. The mucus is a useful protection against the ascent of bacteria and sperm into the uterus. (From Dictionary of Obstetrics and Gynecology, 1988)	Fern Test,Palm-Leaf Reaction,Cervical Mucus,Fern Tests,Mucus, Cervical,Mucus, Cervix,Palm Leaf Reaction,Palm-Leaf Reactions,Reaction, Palm-Leaf,Reactions, Palm-Leaf,Test, Fern,Tests, Fern
D005260	Female		Females
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000372	Agglutination Tests	Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)	Agglutination Test,Test, Agglutination,Tests, Agglutination

Related Publications

M Cerasaro, and A Lenzi, and A Massacesi, and F Dondero

Immunoglobulin class of sperm antibodies in cervical mucus from infertile women.

December 1982, American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction,

M Cerasaro, and A Lenzi, and A Massacesi, and F Dondero

Sperm antibodies in the cervical mucus and sera and postcoital tests in infertile women.

December 1978, European journal of obstetrics, gynecology, and reproductive biology,

M Cerasaro, and A Lenzi, and A Massacesi, and F Dondero

Spermagglutinating antibodies and sperm penetration of cervical mucus from infertile women with spermagglutinating antibodies in serum.

December 1980, Fertility and sterility,

M Cerasaro, and A Lenzi, and A Massacesi, and F Dondero

[The level of immunoglobulins and antispermatic antibodies in the cervical mucus of infertile women with cervix uteri pathology].

January 1997, Likars'ka sprava,

M Cerasaro, and A Lenzi, and A Massacesi, and F Dondero

[Local sperm antibodies and general levels of immunoglobulins in ovulatory cervical mucus].

August 2010, Ceska gynekologie,