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Use of a new synthetic substrate, Nalpha-tosyl-L-arginine-P-nitroanilide (TAPA), for determining trypsin activity in the duodenal contents. 1977

F Malis, and E Kasafírek, and P Fric, and M Josífko

The newly synthesized chromogenic substrate Nalpha-tosyl-L-arginine-p-nitroanilide (TAPA) was used to determine the trypsin activity (as the concentration and output in 20-min samples) of the duodenal contents before and after hormonal stimulation with cholecystokinin-pancreozymin (CPZ) and secretin (S). The results were compared with those obtained by means of Nalpha-benzoyl-D,L-arginine-p-nitroanilide (BAPA) and the Nalpha-tosyl-L-arginine methylester (TAME). The synthetic substrates were compared in 17 subjects with normal and in 8 subjects with pathological CPZ-S test values. A high degree of correlation was found in every case between the values obtained by means of the given substrates and it actually proved possible to determine the conversion coefficients for the trypsin concentration (BAPA = 1.306 TAPA, TAME = 0.509 TAPA) and for trypsin output (BAPA = 1.306 TAPA, TAME = 0.535 TAPA). The results demonstrate the usefulness of our new chromogenic substrate TAPA, which is more readily available and cheaper than BAPA and TAME for determining trypsin activity in biological material.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008297 Male Males
D004386 Duodenum The shortest and widest portion of the SMALL INTESTINE adjacent to the PYLORUS of the STOMACH. It is named for having the length equal to about the width of 12 fingers. Duodenums
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000813 Anilides Any aromatic amide obtained by acylation of aniline.
D001120 Arginine An essential amino acid that is physiologically active in the L-form. Arginine Hydrochloride,Arginine, L-Isomer,DL-Arginine Acetate, Monohydrate,L-Arginine,Arginine, L Isomer,DL Arginine Acetate, Monohydrate,Hydrochloride, Arginine,L Arginine,L-Isomer Arginine,Monohydrate DL-Arginine Acetate
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D014105 Tosyl Compounds Toluenesulfonyl compounds with general formula CH3C6H4S(O2)R” widely used to block amino groups in the course of syntheses of drugs and other biologically active compounds. Toluenesulfonyl Compounds,Compounds, Toluenesulfonyl,Compounds, Tosyl

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