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Affinity chromatography of glycosidases. 1977

M Ledvina, and J Kraml, and J Kolínská

The authors studied the separation of glucoamylase and beta-galactosidases from the interstinal mucosa of young rats by affinity chromatography. They tested the following chromatographic materials: p-aminophenyl-beta-D-thioglucoside bound to Sepharose 4B via hexamethylenediamine, gluconate and galactonate bound in different ways to Sepharose 4B and phlorizin bound by an azo-coupling reaction to a spacer attached to Sepharose 4B. The conditions of the adsorption of glycosidases to these materials and their subsequent elution were studied. Using chromatography on Sepharose 4B with a beta-thioglucoside affinant, we succeeded in purifying lactase preparation so that, in electrophoresis on polyacrylamide gel, it formed a single zone identical with 1-naphthyl-beta-glucosidase activity.

UI MeSH Term Description Entries
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D005960 Glucosides A GLYCOSIDE that is derived from GLUCOSE. Glucoside
D006026 Glycoside Hydrolases Any member of the class of enzymes that catalyze the cleavage of the glycosidic linkage of glycosides and the addition of water to the resulting molecules. Endoglycosidase,Exoglycosidase,Glycohydrolase,Glycosidase,Glycosidases,Glycoside Hydrolase,Endoglycosidases,Exoglycosidases,Glycohydrolases,Hydrolase, Glycoside,Hydrolases, Glycoside

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